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KMID : 0921620100400030123
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Journal of Bacteriology and Virology
2010 Volume.40 No. 3 p.123 ~ p.130
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Mouse Dual Ig Domain Containing Cell Adhesion Molecule Protein Expression and Purification Using the Baculovirus Expression Vector System
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Park Seung-Won
Choi Ji-Hyun
Goo Tae-Won
Kim Seong-Ryul
Lee Gwang-Gil
Kang Seok-Woo
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Abstract
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baculovirus expression vector system (BEVS) is used routinely to produce recombinant proteins in the milligram scale. Dual Ig domain containing cell adhesion molecule (DICAM) belongs to the type I class of transmembrane proteins. It consists of a signal peptide, two V-type Ig domains in the extracellular region, and a short cytoplasmic tail of 442 amino acids. To purify the recombinant DICAM protein from cells overexpressing the mouse full-length DICAM gene, recombinant baculovirus is infected and recovered in the Sf9 cells. As a result, mouse DICAM protein was efficiently expressed and extracted from the insect cells using the BEVS. This recombinant protein can be used in further studies for functional test of DICAM protein in the cells.
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KEYWORD
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Baculovirus expression vector system, Dual Ig domain containing cell adhesion molecule, Recombinant protein
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